Non-homologous end joining as an important mutagenic process in cell cycle-arrested cells
نویسندگان
چکیده
منابع مشابه
Development of an assay to measure mutagenic non-homologous end-joining repair activity in mammalian cells
Double-strand break (DSB) repair pathways are critical for the maintenance of genomic integrity and the prevention of tumorigenesis in mammalian cells. Here, we present the development and validation of a novel assay to measure mutagenic non-homologous end-joining (NHEJ) repair in living cells, which is inversely related to canonical NHEJ and is based on the sequence-altering repair of a single...
متن کاملModeling non-homologous end joining.
Non-homologous end joining (NHEJ) is an important DNA repair pathway for DNA double-strand breaks. Several proteins, including Ku, DNA-PKcs, Artemis, XRCC4/Ligase IV and XLF, are involved in the NHEJ for the DNA damage detection, DNA free end processing and ligation. The classical model of NHEJ is a sequential model in which DNA-PKcs is first recruited by the Ku bound DNA prior to any other rep...
متن کامل[Non-homologous DNA end joining].
DNA double strand breaks (DSB) are the most serious form of DNA damage. Repair of DSBs is important to prevent chromosomal fragmentation, translocations and deletions. Non-homologous end joining (NHEJ) is one of three major pathways for the repair of DSBs in human cells. In this process two DNA ends are joined directly, usually with no sequence homology, although in the case of same polarity of...
متن کاملNon-homologous end joining as a mechanism of DNA repair
In spite of its essential role as the carrier of genetic information, DNA is not an inert structure. The genome is susceptible to potentially mutagenic threats of both endogenous and environmental origin. A dramatic threat to the covalent structure of DNA is posed by breaks in the phosphate backbone affecting one or both strands of the Watson–Crick double helix. Ionizing radiation and certain c...
متن کاملEctopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining
DNA double strand breaks (DSBs) formed during S phase are preferentially repaired by homologous recombination (HR), whereas G1 DSBs, such as those occurring during immunoglobulin class switch recombination (CSR), are repaired by non-homologous end joining (NHEJ). The DNA damage response proteins 53BP1 and BRCA1 regulate the balance between NHEJ and HR. 53BP1 promotes CSR in part by mediating sy...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2003
ISSN: 1460-2075
DOI: 10.1093/emboj/cdg203